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A rapid and versatile method for the isolation, purification and cryogenic storage of Schwann cells from adult rodent nerves
We herein developed a protocol for the rapid procurement of adult nerve-derived Schwann cells (SCs) that was optimized to implement an immediate enzymatic dissociation of fresh nerve tissue while maintaining high cell viability, improving yields and minimizing fibroblast and myelin contamination. Th...
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| Veröffentlicht in: | Sci Rep |
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| Hauptverfasser: | , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4994039/ https://ncbi.nlm.nih.gov/pubmed/27549422 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep31781 |
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