One-Tube-Only Standardized Site-Directed Mutagenesis: An Alternative Approach to Generate Amino Acid Substitution Collections

Site-directed mutagenesis (SDM) is a powerful tool to create defined collections of protein variants for experimental and clinical purposes, but effectiveness is compromised when a large number of mutations is required. We present here a one-tube-only standardized SDM approach that generates compreh...

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Detalhes bibliográficos
Publicado no:PLoS One
Main Authors: Mingo, Janire, Erramuzpe, Asier, Luna, Sandra, Aurtenetxe, Olaia, Amo, Laura, Diez, Ibai, Schepens, Jan T. G., Hendriks, Wiljan J. A. J., Cortés, Jesús M., Pulido, Rafael
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2016
Assuntos:
Research Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4993582/
https://ncbi.nlm.nih.gov/pubmed/27548698
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0160972
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