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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages

A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, th...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Jin, Xueting, Kruth, Howard S.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4993314/
https://ncbi.nlm.nih.gov/pubmed/27404952
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54244
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