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Culture of Macrophage Colony-stimulating Factor Differentiated Human Monocyte-derived Macrophages
A protocol is presented for cell culture of macrophage colony-stimulating factor (M-CSF) differentiated human monocyte-derived macrophages. For initiation of experiments, fresh or frozen monocytes are cultured in flasks for 1 week with M-CSF to induce their differentiation into macrophages. Then, th...
Gardado en:
| Publicado en: | J Vis Exp |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
MyJove Corporation
2016
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4993314/ https://ncbi.nlm.nih.gov/pubmed/27404952 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54244 |
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