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Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Circulating (of cell-free) microRNAs (miRNAs) are released from cells into the blood stream. The amount of specific microRNAs in the circulation has been linked to a disease state and has the potential to be used as disease biomarker. A sensitive and accurate method for circulating microRNA quantifi...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Ferracin, Manuela, Salamon, Irene, Lupini, Laura, Miotto, Elena, Sabbioni, Silvia, Negrini, Massimo
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4993260/
https://ncbi.nlm.nih.gov/pubmed/27403944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54102
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