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Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Maintenance of steady-state calcium (Ca(2+)) levels in the sarcoplasmic reticulum (SR) of vascular smooth muscle cells (VSMCs) is vital to their overall health. A significant portion of intracellular Ca(2+) content is found within the SR stores in VSMCs. As the only intracellular organelle with a cl...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Ziomek, Gabriela, van Breemen, Cornelis, Esfandiarei, Mitra
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4993246/
https://ncbi.nlm.nih.gov/pubmed/27403723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53912
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