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Measurement of Calcium Fluctuations Within the Sarcoplasmic Reticulum of Cultured Smooth Muscle Cells Using FRET-based Confocal Imaging

Maintenance of steady-state calcium (Ca(2+)) levels in the sarcoplasmic reticulum (SR) of vascular smooth muscle cells (VSMCs) is vital to their overall health. A significant portion of intracellular Ca(2+) content is found within the SR stores in VSMCs. As the only intracellular organelle with a cl...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Veröffentlicht in:J Vis Exp
Hauptverfasser: Ziomek, Gabriela, van Breemen, Cornelis, Esfandiarei, Mitra
Format: Artigo
Sprache:Inglês
Veröffentlicht: MyJove Corporation 2016
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4993246/
https://ncbi.nlm.nih.gov/pubmed/27403723
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53912
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