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Use of the melting curve assay as a means for high-throughput quantification of Illumina sequencing libraries

Background. Multiplexed sequencing is commonly performed on massively parallel short-read sequencing platforms such as Illumina, and the efficiency of library normalisation can affect the quality of the output dataset. Although several library normalisation approaches have been established, none are...

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Bibliographic Details
Published in:PeerJ
Main Authors: Shinozuka, Hiroshi, Forster, John W.
Format: Artigo
Language:Inglês
Published: PeerJ Inc. 2016
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Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4991867/
https://ncbi.nlm.nih.gov/pubmed/27602269
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.2281
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