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Protein Quantification by Derivatization-Free High-Performance Liquid Chromatography of Aromatic Amino Acids

Amino acid analysis is considered to be the gold standard for quantitative peptide and protein analysis. Here, we would like to propose a simple HPLC/UV method based on a reversed-phase separation of the aromatic amino acids tyrosine (Tyr), phenylalanine (Phe), and optionally tryptophan (Trp) withou...

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Detalhes bibliográficos
Publicado no:J Amino Acids
Main Authors: Hesse, Almut, Weller, Michael G.
Formato: Artigo
Idioma:Inglês
Publicado em: Hindawi Publishing Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4983383/
https://ncbi.nlm.nih.gov/pubmed/27559481
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1155/2016/7374316
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