Heterogeneous loss of HIV transcription and proviral DNA from 8E5/LAV lymphoblastic leukemia cells revealed by RNA FISH:FLOW analyses

8E5/LAV cells harbor a single HIV provirus, and are used frequently to generate standards for HIV genome quantification. Using flow cytometry-based in situ mRNA hybridization validated by qPCR, we find that different batches of 8E5 cells contain varying numbers of cells lacking viral mRNA and/or vir...

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Détails bibliographiques
Publié dans:Retrovirology
Auteurs principaux: Wilburn, Kaley M., Mwandumba, Henry C., Jambo, Kondwani C., Boliar, Saikat, Solouki, Sabrina, Russell, David G., Gludish, David W.
Format: Artigo
Langue:Inglês
Publié: BioMed Central 2016
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Correspondence
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4982266/
https://ncbi.nlm.nih.gov/pubmed/27515378
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12977-016-0289-2
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