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Genome modifications and cloning using a conjugally transferable recombineering system
The genetic modification of primary bacterial disease isolates is challenging due to the lack of highly efficient genetic tools. Herein we describe the development of a modified PCR-based, λ Red-mediated recombineering system for efficient deletion of genes in Gram-negative bacteria. A series of con...
Gorde:
| Argitaratua izan da: | Biotechnol Rep (Amst) |
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| Egile Nagusiak: | , , , , |
| Formatua: | Artigo |
| Hizkuntza: | Inglês |
| Argitaratua: |
Elsevier
2015
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| Gaiak: | |
| Sarrera elektronikoa: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4980705/ https://ncbi.nlm.nih.gov/pubmed/28352570 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.btre.2015.08.005 |
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