Evaluation of a New Automated, Standardized Generic Nucleic Acid Extraction Method (Total Nucleic Acid Isolation Kit) Used in Combination with Cytomegalovirus DNA Quantification by COBAS AMPLICOR CMV MONITOR

The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. The kit provides a reliable, standardized,...

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Main Authors: Gärtner, Barbara Christine, Fischinger, Johannes Michael, Litwicki, Anke, Roemer, Klaus, Mueller-Lantzsch, Nikolaus
格式: Artigo
語言:Inglês
出版: American Society for Microbiology 2004
主題:
Virology
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC497653/
https://ncbi.nlm.nih.gov/pubmed/15297555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.8.3881-3882.2004
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