Evaluation of a New Automated, Standardized Generic Nucleic Acid Extraction Method (Total Nucleic Acid Isolation Kit) Used in Combination with Cytomegalovirus DNA Quantification by COBAS AMPLICOR CMV MONITOR

The new generic Total Nucleic Acid Isolation kit improved the detection limit of a cytomegalovirus (CMV) PCR from 400 to 200 copies/ml. Sensitivity and specificity in 30 CMV-positive and 100 negative samples were 100%. Analytical performance was excellent. The kit provides a reliable, standardized,...

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Enregistré dans:
Détails bibliographiques
Auteurs principaux: Gärtner, Barbara Christine, Fischinger, Johannes Michael, Litwicki, Anke, Roemer, Klaus, Mueller-Lantzsch, Nikolaus
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology 2004
Sujets:
Virology
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC497653/
https://ncbi.nlm.nih.gov/pubmed/15297555
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.8.3881-3882.2004
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