Comparison of Sequence Analysis and a Novel Discriminatory Real-Time PCR Assay for Detection and Quantification of Lamivudine-Resistant Hepatitis B Virus Strains

We report a rapid and accurate real-time PCR-based method to quantify wild-type and lamivudine-resistant hepatitis B virus by using a common forward primer paired with different reverse primers. Excellent concordance was demonstrated between sequencing and the discriminatory real-time assay; however...

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Detalhes bibliográficos
Main Authors: Wightman, Fiona, Walters, Tomos, Ayres, Anna, Bowden, Scott, Bartholomeusz, Angeline, Lau, Daryl, Locarnini, Stephen, Lewin, Sharon R.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Virology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC497642/
https://ncbi.nlm.nih.gov/pubmed/15297535
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.8.3809-3812.2004
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