Use of 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis for Analysis of the Microfloras of Healing and Nonhealing Chronic Venous Leg Ulcers

The bacterial microfloras of 8 healing and 10 nonhealing chronic venous leg ulcers were compared by using a combination of cultural analysis and denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S rRNA gene products. Cultural analysis of the microflora revealed that the majority of b...

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Detalhes bibliográficos
Main Authors: Davies, Charlotte E., Hill, Katja E., Wilson, Melanie J., Stephens, Phil, Hill, C. Michael, Harding, Keith G., Thomas, David W.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Bacteriology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC497624/
https://ncbi.nlm.nih.gov/pubmed/15297496
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.8.3549-3557.2004
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