Refinement of the Diatom Episome Maintenance Sequence and Improvement of Conjugation-Based DNA Delivery Methods

Conjugation of episomal plasmids from bacteria to diatoms advances diatom genetic manipulation by simplifying transgene delivery and providing a stable and consistent gene expression platform. To reach its full potential, this nascent technology requires new optimized expression vectors and a deeper...

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Udgivet i:Front Bioeng Biotechnol
Main Authors: Diner, Rachel E., Bielinski, Vincent A., Dupont, Christopher L., Allen, Andrew E., Weyman, Philip D.
Format: Artigo
Sprog:Inglês
Udgivet: Frontiers Media S.A. 2016
Fag:
Bioengineering and Biotechnology
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4976089/
https://ncbi.nlm.nih.gov/pubmed/27551676
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fbioe.2016.00065
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