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Rapid and Specific Detection of the O15:K52:H1 Clonal Group of Escherichia coli by Gene-Specific PCR

Primers specific for Escherichia coli O15:K52:H1 were devised based on a novel single-nucleotide polymorphism identified within the housekeeping gene fumC, i.e., G594A. In experiments comparing various reference typing methods, the new primers provided 100% sensitivity and specificity for the O15:K5...

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Autori principali: Johnson, James R., Owens, Krista, Sabate, Montse, Prats, Guillem
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC497591/
https://ncbi.nlm.nih.gov/pubmed/15297544
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/JCM.42.8.3841-3843.2004
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