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A Modular, Tn7-Based System for Making Bioluminescent or Fluorescent Salmonella and Escherichia coli Strains

Our goal was to develop a robust tagging method that can be used to track bacterial strains in vivo. To address this challenge, we adapted two existing systems: a modular plasmid-based reporter system (pCS26) that has been used for high-throughput gene expression studies in Salmonella and Escherichi...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Shivak, Dylan J., MacKenzie, Keith D., Watson, Nikole L., Pasternak, J. Alex, Jones, Brian D., Wang, Yejun, DeVinney, Rebekah, Wilson, Heather L., Surette, Michael G., White, Aaron P.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4968541/
https://ncbi.nlm.nih.gov/pubmed/27260360
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01346-16
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