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Detection of Borrelia burgdorferi in patients with Lyme disease by the polymerase chain reaction.

Borrelia burgdorferi, the causative agent of Lyme disease, was detected in patients' serum by DNA amplification using the polymerase chain reaction (PCR). B burgdorferi was pelleted from serum samples by centrifugation (10,000 x g for 10 minutes) and lysed by treatment with ammonium hydroxide (100 d...

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Библиографические подробности
Опубликовано в::J Clin Pathol
Главные авторы: Guy, E C, Stanek, G
Формат: Artigo
Язык:Inglês
Опубликовано: BMJ Publishing Group 1991
Предметы:
Online-ссылка:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC496808/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1856296/
https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.44.7.610
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