Latitude in sample handling and storage for infant faecal microbiota studies: the elephant in the room?

BACKGROUND: In this manuscript, we investigate the “stones best left unturned” of sample storage and preparation and their implications for the next-generation sequencing of infant faecal microbial communities by the 16S ribosomal ribonucleic acid (rRNA) gene. We present a number of experiments that...

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Detalhes bibliográficos
Publicado no:Microbiome
Main Authors: Shaw, Alexander G., Sim, Kathleen, Powell, Elizabeth, Cornwell, Emma, Cramer, Teresa, McClure, Zoë E., Li, Ming-Shi, Kroll, J. Simon
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Research
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4967342/
https://ncbi.nlm.nih.gov/pubmed/27473284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s40168-016-0186-x
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