Model system for optimising mRNA non-isotopic in situ hybridisation: riboprobe detection of lysozyme mRNA in archival gut biopsy specimens.

The aim of this study was to optimise conditions for mRNA detection by nonisotopic in situ hybridisation (NISH) using biotinylated and digoxigenin labelled riboprobes. Because lysozyme gene transcripts are present at high concentrations in Paneth and other alimentary cells, archival gut biopsy speci...

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Pubblicato in:	J Clin Pathol
Autori principali:	Martinez-Montero, J C, Herrington, C S, Stickland, J, Sawyer, H, Evans, M, Flannery, D M, McGee, J O
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	BMJ Publishing Group 1991
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC496669/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1960217/ https://ncbi.nlm.nih.govhttps://doi.org/10.1136/jcp.44.10.835
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