A reliable and economical method for gaining mouse embryonic fibroblasts capable of preparing feeder layers

Mouse embryonic fibroblasts (MEFs) are widely used to prepare feeder layers for culturing embryonic stem cells (ESCs) or induced pluripotent stem cells (iPSCs) in vitro. Transportation lesions and exorbitant prices make the commercially obtained MEFs unsuitable for long term research. The aim of pre...

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Detalhes bibliográficos
Publicado no:Cytotechnology
Main Authors: Jiang, Guangming, Wan, Xiaoju, Wang, Ming, Zhou, Jianhua, Pan, Jian, Wang, Baolong
Formato: Artigo
Idioma:Inglês
Publicado em: Springer Netherlands 2015
Assuntos:
Method in Cell Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4960116/
https://ncbi.nlm.nih.gov/pubmed/25597023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10616-014-9815-z
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