A continuous spectrophotometric assay that distinguishes between phospholipase A(1) and A(2) activities

A new spectrophotometric assay was developed to measure, continuously and specifically, phospholipase A(1) (PLA(1)) or phospholipase A(2) (PLA(2)) activities using synthetic glycerophosphatidylcholines (PCs) containing α-eleostearic acid, either at the sn-1 position [1-α-eleostearoyl-2-octadecyl-rac...

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Dettagli Bibliografici
Pubblicato in:J Lipid Res
Autori principali: El Alaoui, Meddy, Soulère, Laurent, Noiriel, Alexandre, Popowycz, Florence, Khatib, Abdallah, Queneau, Yves, Abousalham, Abdelkarim
Natura: Artigo
Lingua:Inglês
Pubblicazione: The American Society for Biochemistry and Molecular Biology 2016
Soggetti:
Methods
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4959851/
https://ncbi.nlm.nih.gov/pubmed/27194811
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1194/jlr.D065961
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