One-step generation of cytoplasmic male sterility by fusion of mitochondrial-inactivated tomato protoplasts with nuclear-inactivated Solanum protoplasts.

Mesophyll protoplasts of Lycopersicon esculentum were treated with iodoacetamide to inactivate mitochondria, and protoplasts of Solanum acaule and Solanum tuberosum were irradiated with gamma- or x-rays to inactivate nuclei. Mixtures of protoplasts thus modified were treated with Ca2+ and polyethyle...

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Autores principales: Melchers, G, Mohri, Y, Watanabe, K, Wakabayashi, S, Harada, K
Formato: Artigo
Lenguaje:Inglês
Publicado: 1992
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC49598/
https://ncbi.nlm.nih.gov/pubmed/11607310
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