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Functional Metagenomics of a Biostimulated Petroleum-Contaminated Soil Reveals an Extraordinary Diversity of Extradiol Dioxygenases

A metagenomic library of a petroleum-contaminated soil was constructed in a fosmid vector that allowed heterologous expression of metagenomic DNA. The library, consisting of 6.5 Gb of metagenomic DNA, was screened for extradiol dioxygenase (Edo) activity using catechol and 2,3-dihydroxybiphenyl as t...

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Detalhes bibliográficos
Publicado no:Appl Environ Microbiol
Main Authors: Terrón-González, Laura, Martín-Cabello, Guadalupe, Ferrer, Manuel, Santero, Eduardo
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4959483/
https://ncbi.nlm.nih.gov/pubmed/26896130
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03811-15
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