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Simultaneous in situ hybridisation of native mRNA and immunoglobulin detection by conventional immunofluorescence in paraffin wax embedded sections.

AIMS: The development of a technique for simultaneous in situ hybridisation for native mRNA and conventional immunofluorescence for cytoplasmic antigens in routine pathology specimens. METHODS: Cocktails of synthetic deoxyoligonucleotides coding for immunoglobulin J chain and kappa light chain were...

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Bibliographic Details
Main Authors: Harper, S. J., Pringle, J. H., Gillies, A., Allen, A. C., Layward, L., Feehally, J., Lauder, I.
Format: Artigo
Language:Inglês
Published: 1992
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC495648/
https://ncbi.nlm.nih.gov/pubmed/1541690
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