Introducing mutations into the single-copy chromosomal 23S rRNA gene of the archaeon Halobacterium halobium by using an rRNA operon-based transformation system.

A vector-transformation system is described that permits replacement of a portion of the single rRNA operon of the archaeon Halobacterium halobium with a homologous fragment from a vector-borne gene. The vector construct contains three functional sections: (i) an entire H. halobium rRNA operon with...

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Main Authors: Mankin, A S, Zyrianova, I M, Kagramanova, V K, Garrett, R A
Formato: Artigo
Idioma:Inglês
Publicado: 1992
Assuntos:
Research Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC49536/
https://ncbi.nlm.nih.gov/pubmed/1631155
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