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Imaging Real-Time Gene Expression in Living Systems with Single-Transcript Resolution: Image Analysis of Single mRNA Transcripts

This protocol describes a method for establishing a green fluorescent protein (GFP) calibration curve using dilutions of recombinant GFP and blue fluorescent beads. The total fluorescence intensity (TFI) per mRNA molecule is first calculated by imaging serial dilutions of purified enhanced GFP (eGFP...

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Detalhes bibliográficos
Publicado no:CSH Protoc
Main Authors: Wells, Amber L., Condeelis, John S., Singer, Robert H., Zenklusen, Daniel
Formato: Artigo
Idioma:Inglês
Publicado em: 2007
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4947526/
https://ncbi.nlm.nih.gov/pubmed/21356979
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