Efficient production of (2)H, (13)C, (15)N-enriched industrial enzyme Rhizopus chinensis lipase with native disulfide bonds

BACKGROUND: In order to use most modern methods of NMR spectroscopy to study protein structure and dynamics, isotope-enriched protein samples are essential. Especially for larger proteins (>20 kDa), perdeuterated and Ile (δ1), Leu, and Val methyl-protonated protein samples are required for suppre...

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Bibliografiska uppgifter
I publikationen:Microb Cell Fact
Huvudupphovsmän: Zhang, Meng, Yu, Xiao-Wei, Swapna, G. V. T., Xiao, Rong, Zheng, Haiyan, Sha, Chong, Xu, Yan, Montelione, Gaetano T.
Materialtyp: Artigo
Språk:Inglês
Publicerad: BioMed Central 2016
Ämnen:
Research
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC4944435/
https://ncbi.nlm.nih.gov/pubmed/27411547
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12934-016-0522-7
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