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Selective local lysis and sampling of live cells for nucleic acid analysis using a microfluidic probe

Heterogeneity is inherent to biology, thus it is imperative to realize methods capable of obtaining spatially-resolved genomic and transcriptomic profiles of heterogeneous biological samples. Here, we present a new method for local lysis of live adherent cells for nucleic acid analyses. This method...

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Detalhes bibliográficos
Publicado no:Sci Rep
Main Authors: Kashyap, Aditya, Autebert, Julien, Delamarche, Emmanuel, Kaigala, Govind V.
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4944176/
https://ncbi.nlm.nih.gov/pubmed/27411740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep29579
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