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3′-UTR engineering to improve soluble expression and fine-tuning of activity of cascade enzymes in Escherichia coli
3′-Untranslated region (3′UTR) engineering was investigated to improve solubility of heterologous proteins (e.g., Baeyer-Villiger monooxygenases (BVMOs)) in Escherichia coli. Insertion of gene fragments containing putative RNase E recognition sites into the 3′UTR of the BVMO genes led to the reducti...
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| Veröffentlicht in: | Sci Rep |
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| Hauptverfasser: | , , , , |
| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Nature Publishing Group
2016
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4942690/ https://ncbi.nlm.nih.gov/pubmed/27406241 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep29406 |
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