Endonuclease Restriction-Mediated Real-Time Polymerase Chain Reaction: A Novel Technique for Rapid, Sensitive and Quantitative Detection of Nucleic-Acid Sequence

The article reported a novel methodology for real-time PCR analysis of nucleic acids, termed endonuclease restriction-mediated real-time polymerase chain reaction (ET-PCR). Just like PCR, ET-PCR only required one pair of primers. A short sequence, which was recognized by restriction enzyme BstUI, wa...

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Detalhes bibliográficos
Publicado no:Front Microbiol
Main Authors: Wang, Yi, Wang, Yan, Zhang, Lu, Li, Machao, Luo, Lijuan, Liu, Dongxin, Li, Hua, Cao, Xiaolong, Hu, Shoukui, Jin, Dong, Xu, Jianguo, Ye, Changyun
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2016
Assuntos:
Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4942466/
https://ncbi.nlm.nih.gov/pubmed/27468284
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fmicb.2016.01104
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