Post Column Derivatization Using Reaction Flow High Performance Liquid Chromatography Columns

A protocol for the use of reaction flow high performance liquid chromatography columns for methods employing post column derivatization (PCD) is presented. A major difficulty in adapting PCD to modern HPLC systems and columns is the need for large volume reaction coils that enable reagent mixing and...

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Vydáno v:J Vis Exp
Hlavní autoři: Jones, Andrew, Pravadali-Cekic, Sercan, Hua, Stanley, Kocic, Danijela, Camenzuli, Michelle, Dennis, Gary, Shalliker, Andrew
Médium: Artigo
Jazyk:Inglês
Vydáno: MyJove Corporation 2016
Témata:
Chemistry
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941998/
https://ncbi.nlm.nih.gov/pubmed/27168419
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53462
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