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Preparation of Single-cell Suspensions for Cytofluorimetric Analysis from Different Mouse Skin Regions

The skin is a barrier organ that interacts with the external environment. Being continuously exposed to potential microbial invasion, the dermis and epidermis home a variety of immune cells in both homeostatic and inflammatory conditions. Tools to obtain skin cell release for cytofluorimetric analys...

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Bibliographic Details
Published in:J Vis Exp
Main Authors: Broggi, Achille, Cigni, Clara, Zanoni, Ivan, Granucci, Francesca
Format: Artigo
Language:Inglês
Published: MyJove Corporation 2016
Subjects:
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941935/
https://ncbi.nlm.nih.gov/pubmed/27166881
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/52589
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