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Quantitative Super-Resolution Imaging with qPAINT using Transient Binding Analysis

Current super-resolution techniques offer unprecedented spatial resolution, but quantitative counting of spatially unresolvable molecules remains challenging. Here, we use the programmable and specific binding of dye-labeled DNA probes to count integer numbers of targets. This method, called quantit...

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Bibliografske podrobnosti
izdano v:Nat Methods
Main Authors: Jungmann, Ralf, Avendaño, Maier S, Dai, Mingjie, Woehrstein, Johannes B, Agasti, Sarit S, Feiger, Zachary, Rodal, Avital, Yin, Peng
Format: Artigo
Jezik:Inglês
Izdano: 2016
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4941813/
https://ncbi.nlm.nih.gov/pubmed/27018580
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3804
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