Comparative Evaluation of Three Homogenization Methods for Isolating Middle East Respiratory Syndrome Coronavirus Nucleic Acids From Sputum Samples for Real-Time Reverse Transcription PCR

BACKGROUND: Real-time reverse transcription PCR (rRT-PCR) of sputum samples is commonly used to diagnose Middle East respiratory syndrome coronavirus (MERS-CoV) infection. Owing to the difficulty of extracting RNA from sputum containing mucus, sputum homogenization is desirable prior to nucleic acid...

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Detalhes bibliográficos
Publicado no:Ann Lab Med
Main Authors: Sung, Heungsup, Yong, Dongeun, Ki, Chang-Seok, Kim, Jae-Seok, Seong, Moon-Woo, Lee, Hyukmin, Kim, Mi-Na
Formato: Artigo
Idioma:Inglês
Publicado em: The Korean Society for Laboratory Medicine 2016
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Original Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4940489/
https://ncbi.nlm.nih.gov/pubmed/27374711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3343/alm.2016.36.5.457
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