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Improving the Sensitivity, Resolution, and Peak Capacity of Gradient Elution in Capillary Liquid Chromatography with Large-Volume Injections by Using Temperature-Assisted On-Column Solute Focusing

Capillary HPLC (cLC) with gradient elution is the separation method of choice for the fields of proteomics and metabolomics. This is due to the complementary nature of cLC flow rates and electrospray or nanospray ionization mass spectrometry (ESI-MS). The small column diameters result in good mass s...

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Detalhes bibliográficos
Publicado no:Anal Chem
Main Authors: Wilson, Rachael E., Groskreutz, Stephen R., Weber, Stephen G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4940048/
https://ncbi.nlm.nih.gov/pubmed/27033165
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/acs.analchem.5b04793
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