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“Calibration-on-the-spot”: How to calibrate an EMCCD camera from its images
In order to count photons with a camera, the camera must be calibrated. Photon counting is necessary, e.g., to determine the precision of localization-based super-resolution microscopy. Here we present a protocol that calibrates an EMCCD camera from information contained in isolated, diffraction-lim...
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| Publicado no: | Sci Rep |
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| Main Authors: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Nature Publishing Group
2016
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4933971/ https://ncbi.nlm.nih.gov/pubmed/27381055 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep28680 |
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