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Enzymatic Activity Assays for Base Excision Repair Enzymes in Cell Extracts from Vertebrate Cells

We previously reported enzymatic activity assays for the base excision repair (BER) enzymes DNA polymerase β (pol β), aprataxin (APTX), and flap endonuclease 1 (FEN1) in cell extracts from Saccharomyces cerevisiae (Çağlayan and Wilson, 2014). Here, we describe a method to prepare cell extracts from...

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Shranjeno v:
Bibliografske podrobnosti
izdano v:Bio Protoc
Main Authors: Çağlayan, Melike, Horton, Julie K., Wilson, Samuel H.
Format: Artigo
Jezik:Inglês
Izdano: 2015
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC4933510/
https://ncbi.nlm.nih.gov/pubmed/27390764
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