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Simultaneous all-optical manipulation and recording of neural circuit activity with cellular resolution in vivo

We describe an all-optical strategy for simultaneously manipulating and recording the activity of multiple neurons with cellular resolution in vivo. Concurrent two-photon optogenetic activation and calcium imaging is enabled by coexpression of a red-shifted opsin and a genetically encoded calcium in...

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Vydáno v:Nat Methods
Hlavní autoři: Packer, Adam M., Russell, Lloyd E., Dalgleish, Henry W.P., Häusser, Michael
Médium: Artigo
Jazyk:Inglês
Vydáno: 2014
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC4933203/
https://ncbi.nlm.nih.gov/pubmed/25532138
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nmeth.3217
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