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Light-sheet microscopy for everyone? Experience of building an OpenSPIM to study flatworm development

BACKGROUND: Selective plane illumination microscopy (SPIM a type of light-sheet microscopy) involves focusing a thin sheet of laser light through a specimen at right angles to the objective lens. As only the thin section of the specimen at the focal plane of the lens is illuminated, out of focus lig...

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Detalhes bibliográficos
Publicado no:BMC Dev Biol
Main Authors: Girstmair, Johannes, Zakrzewski, Anne, Lapraz, François, Handberg-Thorsager, Mette, Tomancak, Pavel, Pitrone, Peter Gabriel, Simpson, Fraser, Telford, Maximilian J.
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4929743/
https://ncbi.nlm.nih.gov/pubmed/27363495
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/s12861-016-0122-0
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