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RNA Purification from Intracellularly Grown Listeria monocytogenes in Macrophage Cells
Analysis of the transcriptome of bacterial pathogens during mammalian infection is a valuable tool for studying genes and factors that mediate infection. However, isolating bacterial RNA from infected cells or tissues is a challenging task, since mammalian RNA mostly dominates the lysates of infecte...
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| Опубликовано в: : | J Vis Exp |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
MyJove Corporation
2016
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4927762/ https://ncbi.nlm.nih.gov/pubmed/27341521 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/54044 |
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