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TRUE Gene Silencing: Screening of a Heptamer-type Small Guide RNA Library for Potential Cancer Therapeutic Agents

TRUE gene silencing (termed after tRNase Z(L)-utilizing efficacious gene silencing) is one of the RNA-directed gene silencing technologies, which utilizes an artificial small guide RNA (sgRNA) to guide tRNA 3′ processing endoribonuclease, tRNase Z(L), to recognize a target RNA. sgRNAs can be taken u...

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Bibliografiske detaljer
Udgivet i:J Vis Exp
Main Authors: Haino, Arisa, Ishikawa, Tatsuya, Seki, Mineaki, Nashimoto, Masayuki
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2016
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4927757/
https://ncbi.nlm.nih.gov/pubmed/27285342
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53879
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