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Isolation of Murine Coronary Vascular Smooth Muscle Cells
While the isolation and culture of vascular smooth muscle cells (VSMCs) from large vessels is well established, we sought to isolate and culture VSMCs from the coronary circulation. Hearts with intact aortic arches were removed and perfused via retrograde Langendorff with digestion solution containi...
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Publicado no: | J Vis Exp |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
MyJove Corporation
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4927744/ https://ncbi.nlm.nih.gov/pubmed/27285607 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53983 |
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