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Isolation of Murine Coronary Vascular Smooth Muscle Cells
While the isolation and culture of vascular smooth muscle cells (VSMCs) from large vessels is well established, we sought to isolate and culture VSMCs from the coronary circulation. Hearts with intact aortic arches were removed and perfused via retrograde Langendorff with digestion solution containi...
Guardat en:
| Publicat a: | J Vis Exp |
|---|---|
| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
MyJove Corporation
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4927744/ https://ncbi.nlm.nih.gov/pubmed/27285607 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53983 |
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