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Isolation of Murine Coronary Vascular Smooth Muscle Cells

While the isolation and culture of vascular smooth muscle cells (VSMCs) from large vessels is well established, we sought to isolate and culture VSMCs from the coronary circulation. Hearts with intact aortic arches were removed and perfused via retrograde Langendorff with digestion solution containi...

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Detalhes bibliográficos
Publicado no:J Vis Exp
Main Authors: Husarek, Kathryn E., Zhang, Xiaojin, McCallinhart, Patricia E., Lucchesi, Pamela A., Trask, Aaron J.
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4927744/
https://ncbi.nlm.nih.gov/pubmed/27285607
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53983
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