Shotgun Proteomics of Tomato Fruits: Evaluation, Optimization and Validation of Sample Preparation Methods and Mass Spectrometric Parameters

An optimized protocol was developed for shotgun proteomics of tomato fruit, which is a recalcitrant tissue due to a high percentage of sugars and secondary metabolites. A number of protein extraction and fractionation techniques were examined for optimal protein extraction from tomato fruits followe...

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Detalhes bibliográficos
Publicado no:Front Plant Sci
Main Authors: Kilambi, Himabindu V., Manda, Kalyani, Sanivarapu, Hemalatha, Maurya, Vineet K., Sharma, Rameshwar, Sreelakshmi, Yellamaraju
Formato: Artigo
Idioma:Inglês
Publicado em: Frontiers Media S.A. 2016
Assuntos:
Plant Science
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4925719/
https://ncbi.nlm.nih.gov/pubmed/27446192
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3389/fpls.2016.00969
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