Sample Size, Library Composition, and Genotypic Diversity among Natural Populations of Escherichia coli from Different Animals Influence Accuracy of Determining Sources of Fecal Pollution

A horizontal, fluorophore-enhanced, repetitive extragenic palindromic-PCR (rep-PCR) DNA fingerprinting technique (HFERP) was developed and evaluated as a means to differentiate human from animal sources of Escherichia coli. Box A1R primers and PCR were used to generate 2,466 rep-PCR and 1,531 HFERP...

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Detalhes bibliográficos
Main Authors: Johnson, LeeAnn K., Brown, Mary B., Carruthers, Ethan A., Ferguson, John A., Dombek, Priscilla E., Sadowsky, Michael J.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
Assuntos:
Public Health Microbiology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC492448/
https://ncbi.nlm.nih.gov/pubmed/15294775
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.8.4478-4485.2004
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