Strategy for Cloning Large Gene Assemblages as Illustrated Using the Phenylacetate and Polyhydroxyalkanoate Gene Clusters

We report an easy procedure for isolating chromosome-clustered genes. By following this methodology, the entire set of genes belonging to the phenylacetic acid (PhAc; 18-kb) pathway as well as those required for the synthesis and mobilization of different polyhydroxyalkanoates (PHAs; 6.4 kb) in Pseu...

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Detalhes bibliográficos
Main Authors: García, Belén, Olivera, Elías R., Sandoval, Ángel, Arias-Barrau, Elsa, Arias, Sagrario, Naharro, Germán, Luengo, José M.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2004
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC492377/
https://ncbi.nlm.nih.gov/pubmed/15294843
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.70.8.5019-5025.2004
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