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Quantitative imaging of lipids in live mouse oocytes and early embryos using CARS microscopy

Mammalian oocytes contain lipid droplets that are a store of fatty acids, whose metabolism plays a substantial role in pre-implantation development. Fluorescent staining has previously been used to image lipid droplets in mammalian oocytes and embryos, but this method is not quantitative and often i...

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Enregistré dans:
Détails bibliographiques
Publié dans:Development
Auteurs principaux: Bradley, Josephine, Pope, Iestyn, Masia, Francesco, Sanusi, Randa, Langbein, Wolfgang, Swann, Karl, Borri, Paola
Format: Artigo
Langue:Inglês
Publié: The Company of Biologists Ltd 2016
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC4920167/
https://ncbi.nlm.nih.gov/pubmed/27151947
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1242/dev.129908
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