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Underestimation of ammonia‐oxidizing bacteria abundance by amplification bias in amoA‐targeted qPCR

Molecular methods to investigate functional groups in microbial communities rely on the specificity and selectivity of the primer set towards the target. Here, using rapid sand filters for drinking water production as model environment, we investigated the consistency of two commonly used quantitati...

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Detalhes bibliográficos
Publicado no:Microb Biotechnol
Main Authors: Dechesne, Arnaud, Musovic, Sanin, Palomo, Alejandro, Diwan, Vaibhav, Smets, Barth F.
Formato: Artigo
Idioma:Inglês
Publicado em: John Wiley and Sons Inc. 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4919994/
https://ncbi.nlm.nih.gov/pubmed/27166579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12366
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