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Underestimation of ammonia‐oxidizing bacteria abundance by amplification bias in amoA‐targeted qPCR

Molecular methods to investigate functional groups in microbial communities rely on the specificity and selectivity of the primer set towards the target. Here, using rapid sand filters for drinking water production as model environment, we investigated the consistency of two commonly used quantitati...

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Dettagli Bibliografici
Pubblicato in:Microb Biotechnol
Autori principali: Dechesne, Arnaud, Musovic, Sanin, Palomo, Alejandro, Diwan, Vaibhav, Smets, Barth F.
Natura: Artigo
Lingua:Inglês
Pubblicazione: John Wiley and Sons Inc. 2016
Soggetti:
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4919994/
https://ncbi.nlm.nih.gov/pubmed/27166579
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1111/1751-7915.12366
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