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Simultaneous multicolor imaging of wide-field epi-fluorescence microscopy with four-bucket detection
We demonstrate simultaneous imaging of multiple fluorophores using wide-field epi-fluorescence microscopy with a monochrome camera. The intensities of the three lasers are modulated by a sinusoidal waveform in order to excite each fluorophore with the same modulation frequency and a different time-d...
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Publicado no: | Biomed Opt Express |
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Main Authors: | , , , , |
Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
Optical Society of America
2016
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4918582/ https://ncbi.nlm.nih.gov/pubmed/27375944 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.002285 |
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