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Simultaneous multicolor imaging of wide-field epi-fluorescence microscopy with four-bucket detection

We demonstrate simultaneous imaging of multiple fluorophores using wide-field epi-fluorescence microscopy with a monochrome camera. The intensities of the three lasers are modulated by a sinusoidal waveform in order to excite each fluorophore with the same modulation frequency and a different time-d...

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Detalhes bibliográficos
Publicado no:Biomed Opt Express
Main Authors: Park, Kwan Seob, Kim, Dong Uk, Lee, Jooran, Kim, Geon Hee, Chang, Ki Soo
Formato: Artigo
Idioma:Inglês
Publicado em: Optical Society of America 2016
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC4918582/
https://ncbi.nlm.nih.gov/pubmed/27375944
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.002285
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