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Simultaneous multicolor imaging of wide-field epi-fluorescence microscopy with four-bucket detection
We demonstrate simultaneous imaging of multiple fluorophores using wide-field epi-fluorescence microscopy with a monochrome camera. The intensities of the three lasers are modulated by a sinusoidal waveform in order to excite each fluorophore with the same modulation frequency and a different time-d...
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| Publicat a: | Biomed Opt Express |
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| Autors principals: | , , , , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Optical Society of America
2016
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC4918582/ https://ncbi.nlm.nih.gov/pubmed/27375944 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1364/BOE.7.002285 |
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