The role of local and remote amino acid substitutions for optimizing fluorescence in bacteriophytochromes: A case study on iRFP

Bacteriophytochromes are promising tools for tissue microscopy and imaging due to their fluorescence in the near-infrared region. These applications require optimization of the originally low fluorescence quantum yields via genetic engineering. Factors that favour fluorescence over other non-radiati...

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Pubblicato in:Sci Rep
Autori principali: Buhrke, David, Velazquez Escobar, Francisco, Sauthof, Luisa, Wilkening, Svea, Herder, Nico, Tavraz, Neslihan N., Willoweit, Mario, Keidel, Anke, Utesch, Tillmann, Mroginski, Maria-Andrea, Schmitt, Franz-Josef, Hildebrandt, Peter, Friedrich, Thomas
Natura: Artigo
Lingua:Inglês
Pubblicazione: Nature Publishing Group 2016
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Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC4916461/
https://ncbi.nlm.nih.gov/pubmed/27329837
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/srep28444
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