Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging

Immunohistochemistry is a commonly used clinical and research lab detection technique for investigating protein expression and localization within tissues. Many semi-quantitative systems have been developed for scoring expression using immunohistochemistry, but inherent subjectivity limits reproduci...

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Udgivet i:J Vis Exp
Main Authors: Bauman, Tyler M., Ricke, Emily A., Drew, Sally A., Huang, Wei, Ricke, William A.
Format: Artigo
Sprog:Inglês
Udgivet: MyJove Corporation 2016
Fag:
Basic Protocol
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC4913774/
https://ncbi.nlm.nih.gov/pubmed/27167094
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/53837
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